Estrogen 17β‑estradiol accelerates the proliferation of uterine junctional zone smooth muscle cells via the let‑7a/Lin28B axis in adenomyosis

The estrogen 17β‑estradiol has been proven to serve an indispensable role in the occurrence and development of adenomyosis (ADS). let‑7a/Lin28B axis can control cell proliferation by acting as a tumor‑inhibiting numerous types cancer. However, its ADS remains unknown. present study aimed i) elucidate let‑7a regulating human uterine junctional zone (JZ) smooth muscle cells (SMCs) ADS, ii) evaluate whether modifies expression levels Lin28B JZ SMCs iii) establish how affects function ADS. A total 36 premenopausal women with were enrolled experimental group 34 without recruited group. Reverse transcription‑quantitative PCR was used level let‑7a, western blotting performed determine levels. Lentiviral null vector, overexpression lentiviral vector GV280 inhibition GV369 infect alter for further functional experiments. Cell Counting Kit‑8 assays conducted results demonstrated that downregulated upregulated compared (P<0.0001). Moreover, lower led faster (P<0.05), affected accelerate (P<0.05). These data suggested collaborates affect